Rajashree Srinivasa*, Shikha Mandloi*, Renu Mishra* and Nasreen Siddiqui**

Department o f Botany, Sri Sathya Sai College for Women, Bhopal-462026 (India) **Department of Botany, Govt. Geetanjali Girls College Bhopal (India)


Tuber extract of Gloriosa superba, Family – Colchicaceae, earlier placed in Liliaceae was tested for in vitro antibacterial studies. The acetone and ethanol extract of tuber were taken for the activity. Two Gram positive bacteria staphylococcus aureus and Bacillus subtilis, two Gram negative bacteria Pseudomonas and Escherichia coli were used in the studies using disc diffusion method. The successive Soxhlet extract of tubers of Gloriosa superba were evaporated to dryness using rotary flash evaporator. Dry residue was dissolved in respective solvents (1 : 10 w/v) and tested for antibacterial activity. The extract displayed overwhelming concentration dependent antibacterial properties inhibiting the growth of Staphylococcus aureus and Bacillus subtilis ,far above that of ampicillin used in concentration of 1.0g/ml. The extract was less sensitive to two Gram negative bacteria in the assay. In our present investigation good zone of inhibition was observed in higher concentration against Staphylococcus aureus. Thus the tuber extract of Gloriosa seems to justify their ethnomedical uses and therefore a potential drug that requires further studies and development.

Key words : Antibacterial, Gloriosa superba, Gram positive, Gram negative, acetone, ethanol, disc diffusion, Staphylococcus, Bacillus, Pseudomonas, Escherichia coli.

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